01 Jan Discuss the benefit/lack of benefit there would be for cloning this operon into E coli
that allows the bacteria to break down conta Show more A new operon is discovered in Agrobacterium tumefaciens that allows the bacteria to break down contaminants in the soil. The operon is transcriptionally active only when both contaminants and root exudates are present in the soil. All you know about the operon is that it is in a genomic region of 24kb that is between two restriction sites (NotI and SfiI). The process of contaminant break down happen in stages: 1) the soils pH drops drastically 2) the formation of gooey filaments 3) the formation of a tan powdery substance 4) the formation of distinct while pellets. 1. Assuming that you have a series of BACs spanning the Agrobacterium tumefaciens genome in an overlapping manner describe how you would use mapping techniques to map the position and/or contents of this operon within this 24 kB region. 2. You suspect that this operon has 4 structural genes downstream of regulatory regions. State what regulatory regions you think there are in this operon and how they function justify your answer. How would you design an experiment to characterize what each structural gene does in the operon? 3. Discuss the benefit/lack of benefit there would be for cloning this operon into E. coli. Would it be feasible to clone the operon into E. coli? Why/why not? Show less
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